Top Down Antibody Profiling
Antibody analysis and profiling is a hot topic within the field of proteomics. With our Orbitrap-based mass spectrometers and custom methods, we can provide relative quantitation of antibodies and tandem MS information to confirm its sequence.
We are able to profile mass differences on an intact antibody (and to count the number of modifications). Typical amounts of material required are 50 µL of a 2 pmol/µL solution of each antibody dialyzed against 100 mM ammonium acetate overnight at 4°C.
Related Publications
Fornelli L, Damoc E, Thomas PM, Kelleher NL, Aizikov K, Denisov E, Makarov A, Tsybin YO “Analysis of intact monoclonal antibody IgG1 by electron transfer dissociation Orbitrap FTMS.” Mol Cell Proteomics, 2012, 11(12) p. 1758-67.
Summary | Full Text (PMC) | Full Text (DOI)
Pricing
Northwestern/CBC: Contact us for a quote
LCC: Contact us for a quote
External Academic/Nonprofit: Contact us for a quote
External For-Profit Industry: Contact us for a quote
Instruments Used
Orbitrap Elite
Q Exactive